620-625-Shizong Wong-Guizhi plus F

The relationship between the CaM-CaMK-CREB signal pathway and the treatment for cervical spondylosis by guizhi plus gegen decoction was studied. Group intervention on fibrous ring cells of cervical intervertebral disc of adult rats by medicine serum mixed with Guizhi plus Gegen decoction, blank serum and fetal calf serum respectively was made. Detect by MTT colorimetric assay the growth curve of fibrous ring cell under the adoption of Guizhiplus-Gegen-decoction serum; Conduct western blotting to detect the impact of herbal medicine serum on expression and quantification of CaM, CaMK and CREB; Perform data processing and statistical analysis on the results. The five groups showed no significant difference between each other during the first day after the intervention of Chinese medicine serum(p> 0.05); from the second to seventh day after the intervention, there were significant difference between the blank serum group and other groups(p <0.05). Compared with the blank serum group, Guizhi-plus-Gegen-decoction medicine serum at all doses could substantially improve the expression of CaM, CaMKII, CaMKIV and CREB in fibrous ring cells of cervical intervertebral disc of adult rats (p <0.01), and with the increased doses of Chinese medicine, the capacity of improving the expression level was also increasing. Guizhi-plus-Gegen-decoction medicine serum could highly stimulate the development of fibrous ring cells of cervical intervertebral disc of adult rats. Clinically when it comes to the treatment by Guizhi plus Gegen decoction for cervical spondylosis, there is likely to be an important signal pathway called "CaM-CaMK-CREB".